role of in vitro stimulation with lipopolysaccharide on t-cell activation in hiv-infected antiretroviral-treated patients体外的作用与脂多糖刺激t细胞活化在感染艾滋病毒antiretroviral-treated病人.pdf

Hindawi Publishing Corporation Clinical and Developmental Immunology Volume 2012, Article ID 935425, 9 pages doi:10.1155/2012/935425 Clinical Study Role of In Vitro Stimulation with Lipopolysaccharide on T-Cell Activation in HIV-Infected Antiretroviral-Treated Patients ` Camilla Tincati, Giusi M. Bellistrı, Giuseppe Ancona, Esther Merlini, Antonella d’Arminio Monforte, and Giulia Marchetti Department of Medicine, Surgery and Dentistry, Clinic of Infectious Diseases and Tropical Medicine, “San Paolo” Hospital, University of Milan, Via A. di Rudinı 8, 20142 Milan, Italy ` Correspondence should be addressed to Giulia Marchetti, giulia.marchetti@unimi.it Received 31 August 2011; Revised 8 November 2011; Accepted 10 November 2011 Academic Editor: Carlo Torti Copyright © 2012 Camilla Tincati et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. We investigated the effect of LPS in vitro stimulation on T-cell activation in HIV-infected patients with different CD4+ recovery on HAART. PBMCs from 30 HIV-positive, HAART-treated, aviremic individuals with different CD4+ reconstitution (Low Responders: CD4+ 350/µL; Intermediate Responders: CD4+ 350–599/µL; High Responders: CD4+ ≥ 600/µL) were cultured with LPS and the proportion of HLA-DR/CD38- and Ki67-expressing CD4+/CD8+ T-cells was measured (flow cytometry). Upon LPS stimulation, significantly higher CD4+ and CD8+HLA-DR+ cells were shown in LR and IR versus HIV-negative controls. While no differences in the propo