screening for variants in the mutyh gene in saudis筛查在沙特mutyh基因的变异.pdf

Open Journal of Genetics, 2011, 1, 70-77 OJGen doi:10.4236/ojgen.2011.13013 Published Online December 2011 (http://www.SciRP.org/journal/ojgen/). Screening for variants in the MUTYH gene in Saudis * Jameela Shinwari, Abdulla Alamri, Mohammad Alanazi, Nada Al Tassan Department of Biochemistry, Genome Research Chair, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia. * Email: naltassan@.sa Received 26 September 2011; revised 11 November 2011; accepted 22 November 2011. ABSTRACT pulations. MUTYH is a base excision repair glycosylase re- Keywords: MUTYH ; Allele Frequencies; Single Nuc- sponsible for correcting the G:A mismatches that leotide Polymorphism; Mutations; Colorectal Cancer arise from replication of a damaged DNA, such im- pairment results from attacks by reactive oxygen 1. INTRODUCTION species that are produced from different sources Genomic DNA damage occurs as a result of chemical including cigarette smoking. The produced reactive oxygen species trigger the oxidation of guanine in to events that are triggered by endogenous processes or the 8-oxo-G and the latter mispairs with adenine. Alte- exposure to exogenous substances [1]. The most fre- rations in the MUTYH gene can affect its glycosy- quent DNA damage is caused by Reactive Oxygen Spe- lase function and hence the DNA repair capacity cies (ROS) [2-3]. These species are generated by normal that is t