season, environment stress and refrigerated storage affect genomic dna isolation of tung tree季节,环境压力和冷藏影响基因组dna的隔离东树.pdf

American Journal of Plant Sciences, 2012, 3, 1562-1567 /10.4236/ajps.2012.311188 Published Online November 2012 (http://www.SciRP.org/journal/ajps) Season, Environment Stress and Refrigerated Storage Affect Genomic DNA Isolation of Tung Tree Lingling Zhang1,2,3, Yue Pan1,2,3, Jinmin Fu1,2, Junhua Peng1,2* 1Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, China; 2Key Lab of Plant Germplasm Enhancement and Specialty Agriculture, Chinese Academy of Sciences, Wuhan, China; 3Graduate University of Chinese Academy of Sciences, Beijing, China. * * Email: jpeng@, junhuapeng@ Received August 11th, 2012; revised September 19th, 2012; accepted October 6th, 2012 ABSTRACT Many metabolites in leaf tissue disturbed plant genomic DNA isolation and they always varied when leaf was harvested from different environments. Objective of this study was to investigate whether season, environment stress and refriger- ated storage affect genomic DNA isolation of tung tree leaves. Five types of young leaves and two DNA isolation pro- tocols, the recycling CTAB protocol I and II, were adopted to carry out the experiment. Our results showed that both leaf type and protocol affected DNA isolation of tung tree. Using the recycling CTAB protocol II, though little DNA were obtained from three types of young leaves, though the other two have satisfying results. Whereas the recycling CTAB protocol I could produce high yield genomic DNA from all the five types of young leaves. All the detectable DNA samples in agarose gel electrophoresis were good templates for PCR reaction. Season, environment stress and refrigerated storage had a big effect on genomic DNA isolation of tung tree. The recycling CTAB protocol I was proved to be an effective and universal protocol for DNA isolation of tung tree. Five types of young leaves could all act as the tissue for isolation of genomic DNA, but the summer healthy you