serum interleukin-4 and total immunoglobulin e in nonatopic alopecia areata patients and hla-drb1 typing血清interleukin-4和总免疫球蛋白e nonatopic斑秃患者和hla-drb1打字.pdf

Hindawi Publishing Corporation Dermatology Research and Practice Volume 2010, Article ID 503587, 6 pages doi:10.1155/2010/503587 Clinical Study Serum Interleukin-4 and Total Immunoglobulin E in Nonatopic Alopecia Areata Patients and HLA-DRB1 Typing Enas A. S. Attia,1 Dina El Shennawy,2 and Ashraf Sefin3 1 Department of Dermatology, Venereology, and Andrology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt 2 Department of Clinical Pathology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt 3 Department of Public Health, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt Correspondence should be addressed to Enas A. S. Attia, annosah74@ Received 8 April 2010; Accepted 1 June 2010 Academic Editor: Khusru Asadullah Copyright © 2010 Enas A. S. Attia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Background. Interleukin-4 (IL-4), a Th2 cytokine, can stimulate immunoglobulin E (IgE) transcription. No previous studies evaluated the genetic mechanisms in nonatopic AA patients with elevated serum IgE. Objective. To compare serum IL-4 and total IgE levels between Egyptian nonatopic AA patients and healthy subjects and to investigate a possible relation to HLA-DRB1 alleles. Results. Serum IL-4 and total IgE were measured by ELISA in 40 controls and 54 nonatopic AA patients. Patients’ HLA- DRB1 typing by sequence specific oligonucleotide probe technique was compared to normal Egyptian population. We found significantly elevated serum IL-4 and total IgE in AA patients (particularly alopecia universalis, AU, and chronic patients) (P .01).