stimulation of root growth induced by aluminum in quercus serrata thunb. is related to activity of nitrate reductase and maintenance of iaa concentration in roots刺激根系生长铝诱导的quercus serrata研究。.pdf

American Journal of Plant Sciences, 2012, 3, 1619-1624 1619 /10.4236/ajps.2012.311196 Published Online November 2012 (http://www.SciRP.org/journal/ajps) Stimulation of Root Growth Induced by Aluminum in Quercus serrata Thunb. Is Related to Activity of Nitrate Reductase and Maintenance of IAA Concentration in Roots 1 1 1 1 2 Rie Tomioka , Chisato Takenaka , Masayoshi Maeshima , Takafumi Tezuka , Mikiko Kojima , Hitoshi Sakakibara2 1Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan; 2RIKEN Plant Science Center, Yokohama, Japan. Email: tomiokar@agr.nagoya-u.ac.jp Received August 10th, 2012; revised September 24th, 2012; accepted October 16th, 2012 ABSTRACT Aluminum (Al) is the most abundant metal in the earth’s crust. Excess Al3+ released by soil acidification in soil solution is thought to be a growth limiting factor to many cultivated plant species, but it has been reported to stimulate plant growth in some crop and tree species in certain concentration of Al3+. Previously, we had reported that Al treatment enhanced root development, NO uptake from growth media and in vivo nitrate reductase (NR) activity of roots. NR 3 is one of the key enzymes in nitrogen metabolism and acts at the first step of nitrate assimilation in plants. In this study, we investigated the process of Al-induced root development in an early stage, focusing on the change in in vitro NR activity, and indole-3-acetic acid (IAA) and cytokinins concentration in roots of Quercus serrata seedlings, which were treated for 1 h with Al or Ca. In Al-treated roots, NR activity increased and IAA concentration was maintained at the same level as pretreatment, and indole-3-acetyl-L-aspartic acid (IA-Asp