三叉神经痛大鼠中钙通道α2δ-1亚单位表达变化.docVIP

三叉神经痛大鼠中钙通道α2δ-1亚单位表达变化[摘要] 目的 研究大鼠眶下神经结扎后钙通道α2δ-1亚单位（Cavα2δ-1）表达的变化，探讨Cavα2δ-1与三叉神经痛的关系。方法 将12只SD大鼠随机分为神经结扎组和假手术对照组，每组6只。用铬肠线轻松结扎神经结扎组大鼠的眶下神经，建立三叉神经的神经病理性疼痛动物模型；假手术对照组仅暴露神经，不结扎。术前1 d，术后第3、6、9、12、15天测定机械刺激反应阈值。术后第15天收集三叉神经节（TG）和三叉神经脊束核尾侧亚核及颈段第一、二脊髓背侧角（Vc/C2）组织，采用Western印迹杂交法测定Cavα2δ-1蛋白浓度。结果 神经结扎组大鼠术侧机械刺激反应阈值在术后逐渐降低，与术前比较，术后第9、12、15天机械刺激反应阈值差异有统计学意义（P0.05）。术后第15天神经结扎组术侧TG和Vc/C2中Cavα2δ-1表达明显上调（P0.01）。结论 Cavα2δ-1参与了大鼠三叉神经痛的发生发展，为进一步探讨三叉神经痛发病机制提供实验基础。 [关键词] 钙通道α2δ-1亚单位； 三叉神经痛； 眶下神经结扎 [中图分类号] Q 26 [文献标志码] A [doi] 10.3969/j.issn.1000-1182.2012.03.022 Change of calcium channel alpha-2-delta-1 subunit expression levels in trigeminal neuralgia rats Zhang Xia1，2, Bai Xiaofeng3, Zhang Qian1，2. （1. Dept. of Anesthesiology, School of Stomatology, China Medical University, Shenyang 110002, China; 2. Dept. of Anesthesiology, Liaoning Research Institute of Stomatology, Shenyang 110002, China; 3. Dept. of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, Shenyang 110002, China） [Abstract] Objective To determine the expression levels of calcium channel alpha-2-delta-1（Cavα2δ-1） subunit in trigeminal ganglia（TG） and trigeminal spinal subnucleus caudalis C1-C2 spinal cervical dorsal horn（Vc/C2） after infraorbital nerve（ION） ligation injury in relation to the development of neuropathic nociception. Methods Twelve male SD rats were divided into two groups randomly, with 6 rats for each. In the ligation group, the ION was ligated by chromic gut loosely. In the sham operation group, the ION was exposed under same procedure but not ligated. The behavioral tests were conducted before 1 d and after 3, 6, 9, 12, 15 d with calibrated von Frey filaments. Cavα2δ-1 protein levels in TG and Vc/C2 were measured with Western blots 15 days after surgery. Results Compared with the sham operation group, changes of allodynia to mechanical stimulation on the territory of ligated ION were found from the 9th to 15th day after operation in the ligation group（P0.05）