吸入低浓度CO抑制兔心肌缺血-再灌注后核转录因子CHOP表达.doc

吸入低浓度CO抑制兔心肌缺血-再灌注后核转录因子CHOP表达[摘要] 目的：观察吸入低浓度CO是否能抑制兔心肌缺血-再灌注后核转录因子CHOP的表达。方法：结扎心脏冠脉前降支30 min造成心肌缺血，松开结扎120 min作为再灌注，建立兔心肌缺血-再灌注损伤的实验模型。40只家兔随机分成4组：假手术组（sham组）、缺血-再灌注（I-R）组、CO前处理组和低氧前处理组，每组各10只。除sham组外，其余各组均给予缺血-再灌注损伤处理。I-R组、CO前处理组和低氧前处理组分别于心肌缺血-再灌注术前24 h将家兔饲养于密闭容器内吸入正常空气、250 ppm CO和使密闭容器内维持低氧状态0.5 atm，持续24 h。以Western blot法分析各组心肌中核转录因子CHOP的表达水平。结果：给予250 ppm的CO前处理组家兔的心肌CHOP表达明显低于I-R组；而低氧前处理组没有此作用。结论：吸入低浓度的CO（250 ppm）能明显改善心肌缺血-再灌注损伤；其机制可能为外源性CO缓解缺血-再灌注激发的过度内质网应激（ERS），抑制CHOP过表达，减轻ERS相关细胞凋亡的发生，从而减轻心肌缺血-再灌注损伤。 [关键词] 一氧化碳；心肌缺血-再灌注损伤；血红素加氧酶-1；CHOP；内质网应激 [中图分类号] R364[文献标识码]A [文章编号]1673-7210（2009）05（c）-018-03 Inhalation of low concentration CO suppresses the expression of CHOP in rabbits myocardial ischemia-reperfusion injury model LU Jun, MA Jie, LI Xinhua, YAN Zixing, ZHANG Wenkai (Department of Cardiothoracic Surgery, the Second Affiliated Hospital of Shanxi Medical University, Taiyuan030001, China) [Abstract] Objective: To investigate whether low concentration carbon monoxide (CO) would inhibit the expression of the nuclear transcription factor C/EBP homologous (CHOP) after myocardial ischemia-reperfusion injury in rabbits. Methods: Myocardial ischemia-reperfusion was induced by occluding the left anterior descending coronary artery for 30 minutes and releasing for 120 min. 40 rabbits were randomly divided into 4 groups: sham group, I-R group, CO pretreatment group and hypoxia pretreatment group, with 10 rabbits in each group. Except sham group, other groups were given myocardial I-R injury operation, rabbits in all groups except sham group inhaled normal air, CO (250 ppm) and sufferred hypoxia (0.5 atm) respectively for 24 hours in a chamber before surgical operation. Then nuclear transcription factor expression level of CHOP in each group was detected with Western blot. Results: The CO pretreatment group received pre-exposure to 250 ppm of CO significantly reduced the expression of CHOP in the heart compared with I-R group; however, hypoxia pretreatme