年龄相关性白内障晶状体上皮细胞中hTERT活性表达.docVIP

年龄相关性白内障晶状体上皮细胞中hTERT活性表达[摘要] 目的：探讨hTERT在年龄相关性白内障晶状体上皮细胞（lens epithelial cells,LEC）中的活性表达及在白内障形成中的作用。方法：对40例年龄相关性白内障患者和10例正常LEC中的hTERT进行原位杂交技术检测，并进行比较。结果：年龄相关性白内障LEC中hTERT的阳性表达率明显增高，与正常晶状体组相比，有显著性差异(P＜0.01)。结论：LEC中hTERT活性的变化与年龄相关性白内障的形成密切相关，随着年龄的增长，hTERT的表达活性升高，使LEC发生衰老，从而形成白内障。 [关键词] 端粒酶逆转录酶；晶状体上皮细胞；年龄相关性白内障 [中图分类号] R776.1[文献标识码]A [文章编号]1673-7210（2010）10（a）-019-03 Expression of human telomerase reverse transcriptase in lens epithelial cells of age-related cataract TAO Dan，WANG Xuemei, LEI Huo (Department of Ophthalmology, the First People’s Hospital of Yunnan Province the Affiliated Kunhua Hospital of Kunming Medical College, Kunming 650031, China) [Abstract] Objective: To investigate the expression of human telomerase reverse transcriptase (hTERT) in the lens epithelial cells(LEC)of age-related cataract and its effect on age-related cataract formation． Methods: In situ hybridization was used to detect the mRNA expression of hTERT in 40 LEC specimen of age-related cataract, 10 LEC specimen from normal persons cataracts. Results: The positive rate of hTERT in age-related cataract LEC was significantly higher with the moderate and strong staining intensity in comparison with traumatic LEC (P＜0.01). Conclusion: Over expression of hTERT in age-related cataract LEC is related to cataract. With age the increase of telomerase activity is the mechanism of age-related cataract． [Key words] hTERT; LEC; Age-related cataract 年龄相关性白内障 （age-related cataract，ARC）是最常见的白内障类型，其患病率随年龄的增长而明显增高。据不完全统计在我国50～60岁年龄相关性白内障发病率为60%～70%，70岁以上的达80%，80岁以上的老年人几乎达100%。年龄相关性白内障的病因复杂，其发病机制迄今为止尚未完全达成共识。本研究采用原位杂交的方法检测人端粒酶逆转录酶（human telomerase reverse transcriptase，hTERT）在人LEC中的活性表达，探讨其在年龄相关性白内障发病机制中的作用，为进一步阐明其发病机制提供实验依据。 1 材料与方法 1.1 材料 年龄相关性白内障组晶状体上皮细胞取自2009年3～5月份在我院行白内障超声乳化术的病人40例，其中，男27例，女13例；年龄55～80岁，平均69.8岁。术前裂隙灯显微镜排外其他眼部疾病及全身性疾病。正常人组晶状体上皮细胞取自透明晶状体白内障摘除术的患者10例，其中，男6例，女4例；年龄18～30岁，平均24岁。二者均在术中环形撕囊取前囊膜。 1.2 试剂 兔抗TERT多克隆抗体(BA0564)及人TERT原位杂交检测试剂盒（MK1158-h）均购自武汉博士