实验四 培养基的制备与灭菌（Experiment four preparation and sterilization of culture medium）.docVIP

实验四 培养基的制备与灭菌（Experiment four preparation and sterilization of culture medium） Experiment four preparation and sterilization of culture medium I. requirements 1., through experiments, we should master the culture methods of three different microorganisms, bacteria, actinomycetes and fungi. 2. understand the principle of high pressure steam sterilization, learn dry heat sterilization and high-pressure steam sterilization technology. Two content and steps [preparation of culture medium] Weighing, heating, melting, adjusting pH value, filtering, loading and sterilizing [operation points] 1. heating and melting After weighing, add the amount of water needed (one or more times to water) in the beaker, heat, stir, and dissolve. If the solid medium is prepared, the agar must be constantly stirred and controlled during the melting process. Do not allow the medium to overflow or burn. To be completely melted, make up the loss of water, if the formula contains starch, you must first the starch with a small amount of cold water into a paste and stir heating on the fire, then add enough water and other drugs, to be completely melted to complement the loss of water. 2. adjust the pH value Medium formula for natural pH, without regulation, otherwise it must be adjusted to meet the needs. General practice is: medium dissolved evenly and cooled to room temperature, with pH test paper pH, and then according to the requirements of acid or alkali (usually use 1molHCl and 1molNaOH), to slow, a small amount and stir. 3. packing According to different needs, can be divided into the culture medium prepared in vitro or triangular bottle (bottle), pipe plug of cotton plug. The solid medium must be packed separately when hot. To avoid contamination of the medium, the tube (bottle) mouth can cause pollution when packing. The packing capacity can be divided into the following aspects according to the situation: (1): slope load length 1/4-1/5. (2) the liquid culture medium, high medium, semi so