肥胖者及2型糖尿病患者瘦素基因的表达（Leptin gene expression in obese and type 2 diabetic patients）.docVIP

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肥胖者及2型糖尿病患者瘦素基因的表达（Leptin gene expression in obese and type 2 diabetic patients）.doc

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肥胖者及2型糖尿病患者瘦素基因的表达（Leptin gene expression in obese and type 2 diabetic patients）

肥胖者及2型糖尿病患者瘦素基因的表达（Leptin gene expression in obese and type 2 diabetic patients） Leptin gene expression in obese and type 2 diabetic patients 2010-10-06 Xu Mingtong, Zhong Guangshu, Fu Zuzhi, LV Ling, Cheng Hua Recent studies have shown that leptin (leptin, gene), an old product called ob (gene), is a coding product of leptin (leptin), which plays an important role in weight regulation. In this study, the leptin gene mRNA levels in the subcutaneous adipose tissue of patients with simple obesity and type 2 diabetes mellitus were detected by RNA dot blot hybridization in Chinese Han nationality. I. object 42 cases of abdominal surgery patients, according to body mass index (BMI) 24 as the boundary, diagnostic criteria, and WHO on diabetes is divided into: non obese group (NO): male 6/ female 5, age (51 + 10.2), BMI 21 + 1.5; simple obesity (O) group: male 4/ 8 year old female, (51.8 + 9.3), BMI 28.5 + 2.3; type 2 diabetes in non obese group (DM-NO): male 6/ female 5, age (59.5 + 7.3), BMI 21.2 + 1.4; type 2 diabetes obesity (DM-O) group: male 2/ female 6. Age (58.8 + 8.8), BMI 27 + 1.4. There was no significant difference in age and sex between the groups, and the difference of BMI was significant. Abdominal subcutaneous adipose tissue was removed and stored in liquid nitrogen. Two, methods 1. of the total RNA preparation: total RNA extracted the guscn adipose tissue. 2. the preparation of cDNA probe of leptin gene: the recombinant plasmid pUC19-ob was constructed by BamH, I and EcoR I (DNA), The obtained leptin gene cNDA fragment (640bp) was labeled with digoxin (DIG) using random primers. 3.RNA dot blot hybridization: each RNA sample was transferred to nylon membrane by 15 mu G. After fixation, twelve hours in advance at 50 DEG C for 2 hours in the pre hybridization liquid (hybridization buffer: 7%SDS, 50% formamide, 5 * SSC, 2% closed solution, 50mmol/L sodium phosphate, and 0.1% sodium alkyl sodium alginate). The hybrid solution (2.5ml/100cm2, containing the hybri