清热化瘀方对脑缺血再灌注大鼠GDNF mRNA及其蛋白表达影响1).docVIP

清热化瘀方对脑缺血再灌注大鼠GDNF mRNA及其蛋白表达影响1)摘要：目的观察大鼠脑缺血再灌注后脑内胶质细胞源性神经营养因子(GDNF) mRNA和蛋白的表达变化以及清热化瘀方对其的影响。方法 采用线栓法制作局灶性脑缺血大鼠(MCAO)模型，用原位杂交法和免疫组化法观察脑缺血后 3 h、6 h、12 h及 1 d、3 d、7 d共 6个时间点 GDNF mRNA及其蛋白的表达变化。结果 缺血半暗带GDNF mRNA及其蛋白的表达均于缺血灌注后3 h开始明显上升，6 h达高峰，12 h表达开始减弱(P0.05或P0.01)，3 d后降至正常水平。清热化瘀方治疗后可以上调GDNF的表达。结论 清热化瘀方可促进脑缺血后GDNF的表达，保护神经元。 关键词:清热化瘀方；脑缺血；胶质细胞源性神经营养因子 中图分类号：R743.31 R255.2 文献标识码：A 文章编号：1672 ________________________________________ 1349（2011）09 ________________________________________ 1088 ________________________________________ 02 Effect of Qingre Huayu Prescription on Expression of Glial Cell Line ________________________________________ derived Neurotrophic Fator mRNA and Protein in Rats after Cerebral Ischemia/reperfusion Hu Yueqiang，Tang Nong，Liu Tai，et al The First Affiliated Hospital，Guangxi University of Traditional Chinese Medicine(Nanning 530023) Abstract:Objective To observe the mRNA and protein levels of glial cell line ________________________________________ derived neurotrophic fator(GDNF) in rats after focal cerebral ischemia/reperfusion(I/R) and effect of Qingre Huayu prescription(QHP) on them.Methods The I/R rat models were established by middle cerebral artery occlusion to observe the expression variation of GDNF mRNA and protein at 3 h，6 h，12 h and 1 d，4 d，7 d after I/R by in situ hybridization and immunochemistry method.ResultsThe expression of GDNF mRNA and protein in the penumbra region were increased at 3 h after reperfusion，and peaked at 6 hours，then decreased continuously at 12 h(P0.05 or P0.01).The level of GDNF was increased in QHP group.Conclusion Qingre Huayu prescription could protect neuron through upregulating the expression of GDNF after I/R in rats. Key words: Qingre Huayu prescription；cerebral ischemia；glial cell line ________________________________________ derived neurotrophic fator 脑缺血损伤后，除了存在一种主动性的程序性细胞死亡(凋亡)过程，在耐受细胞内还存在一种平行的主动性神经元存活过程，而神经营养因子就参与了这种主动性神经元存活过程。胶质细胞源性神