牙周致病菌共聚和其对人工牙根面黏附力影响.doc

牙周致病菌共聚和其对人工牙根面黏附力影响[摘要]目的比较具核梭杆菌、牙龈卟啉单胞菌、中间普雷沃菌和伴放线嗜血菌等牙周病致病菌彼此之间的共聚力大小，观察四者在具核梭杆菌介导下对人工牙根面黏附力的影响，了解牙周生物膜结构中细菌间可能存在的相互作用。方法目测具核梭杆菌、牙龈卟啉单胞菌、中间普雷沃菌和伴放线嗜血菌彼此间的共聚力，以放射性核素闪烁计数四者在具核梭杆菌黏附和未黏附状况下对胶原包被羟磷灰石（c-HA）的黏附间是否存在着差异。结果具核梭杆菌、牙龈卟啉单胞菌、中间普雷沃菌和伴放线嗜血菌彼此间存在着共聚作用，其中，具核梭杆菌与牙龈卟啉单胞菌、牙龈卟啉单胞菌与中间普雷沃菌间的共聚度均可达4度，具核梭杆菌与其他三菌间的共聚度均大于3度。牙龈卟啉单胞菌、中间普雷沃菌和伴放线嗜血菌在具核梭杆菌黏附的状况下对c-HA的黏附率高于其在具核梭杆菌未黏附时的黏附率，具核梭杆菌在未黏附的状况下对c-HA的黏附率高于其在黏附后的黏附率。结论具核梭杆菌、牙龈卟啉单胞菌、中间普雷沃菌和伴放线嗜血菌彼此间均存在共聚关系，具核梭杆菌可能对其他牙周病致病菌定植于牙菌斑起到了桥梁作用。 [关键词]牙周致病菌；共聚；黏附；胶原包被羟磷灰石膜 [中图分类号]R 780.2[文献标志码]A[doi]10.3969/j.issn.1673-5749.2012.01.008 Coaggregation of periodontal pathogens and its effect on the attachment on artificial root surfaceWang Yaosheng1, Du Qifeng2, Li Yongkai2, Xu Yi2，3.（1. Dept. of Periodontics, Shanxi Red Cross Stomatological Hospital, Taiyuan 030001, China; 2. State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China; 3. Dept. of Periodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China） [Abstract]ObjectiveThe coaggregation abilities of 4 strains of periodontal pathogens Fusobacterium nucleatum（F.nucleatum）, Porphytomonas gingivalis（P.gingivalis）, Prevotella intermedia（P.intermedia）and Haemophilus actinomycetemcomitans（H.actinomycetemcomitans）were compared. The changes of the ability of periodontal pathogens attaching to artificial root surface mediated by F.nucleatum were observed in order to investigate the possible interaction of the bacteria in periodontal biofilm. MethodsThe coaggregation degree among 4 strains of periodontal pathogens was investigated by a visual assay. Differences of the adherent percent of periodontal pathogens to collagen-coated hydroxyapatite（c-HA）under two conditions when F.nucleatum had already bond to c-HA and when F.nucleatum had not, were detected by radioisotope scintillation counter. ResultsThe coaggregation among 4 studied strains was observed. The coaggregation degrees between F.nucleatum and P.gingivalis, as well as