酚氨咖敏颗粒含量测定方法改进.docVIP

酚氨咖敏颗粒含量测定方法改进[摘要] 目的：建立HPLC法同时测定氨酚咖敏颗粒中对乙酰氨基酚、咖啡因、氨基比林的含量。方法：采用HPLC法，色谱柱：Kromasil C18(4.6 mm×250 mm，5 μm)；流动相：1%醋酸溶液（用三乙胺调pH为3.5）-甲醇-乙腈（65∶10∶25）；流速：1.0 ml/min；检测波长：273 nm；柱温：30℃。结果：对乙酰氨基酚检测浓度的线性范围为12.3～123.2 μg/ml（r=0.999 6），平均回收率为98.65%，RSD=0.31%（n=3）；咖啡因检测浓度的线性范围为2.44～24.42 μg/ml（r=0.999 8），平均回收率为99.15%，RSD=0.15%（n=3）；氨基比林检测浓度的线性范围为8.23～82.34 μg/ml（r=0.999 9），平均回收率为98.40%，RSD=0.39%（n=3）。结论：本法灵敏，可靠，简单可行，可用于氨酚咖敏颗粒中对乙酰氨基酚、咖啡因、氨基比林的含量测定。 [关键词] 氨酚咖敏颗粒；对乙酰氨基酚；咖啡因；氨基比林；HPLC [中图分类号] R927.2 [文献标识码] A [文章编号]1673-7210（2010）11（b）-044-03 Simultaneous determination of Paracetamol, Caffeine, Aminopyrine in Paracetamol, aminophenazone phenacetin, caffeine and chlorphenamine maleate Granules JIAO Fuzhe1, GAO Jianling2 (1.Nanyang Health School for Medical Workers, He’nan Province, Nanyang 473000, China; 2.Nanyang Medical College, He’nan Province, Nanyang 473000, China) [Abstract] Objective: To establish a method for determination of Paracetamol, Caffeine, Aminopyrine in Paracetamol, aminophenazone phenacetin, caffeine and chlorphenamine maleate Granules by HPLC.Methods: The Kromasil C18 column (4.6 mm×250 mm, 5 μm) was used, a mixture of 1%-Acetic acid (adjust pH to 3.5 by triethylamine)-methanol-acetonitrile (65∶10∶25） as the mobile phase. The detection wavelength was at 273 nm, the flow rate was 1.0 ml/min. The temperature of the column was at 30℃. Results: The linear of range Paracetamol was at 12.3-123.2 μg/ml (r=0.999 6), the average recovery was 98.65% with a RSD of 0.31% (n=3); the linear range of Caffeine was 2.44-24.42 μg/ml (r=0.999 8), the average recovery was 99.15% with a RSD of 0.15% (n=3); the linear range of Aminopyrine was 8.23-82.34 μg/ml (r=0.999 9), the average recovery was 98.40% with a RSD of 0.39%(n=3). Conclusion: The method is simple, reliable and sensitive. This method can be used for the determination of Paracetamol, Caffeine, Aminopyrine in Paracetamol, aminophenazone phenacetin, caffeine and chlorphenamine maleate Granules. [Key w