小克银汉菌发酵产γ—亚麻酸的动力学的分析研究.pdfVIP

摘 要 -亚麻酸是多价不饱和脂肪酸的一种 它在人体内可转化为前列腺素和白三烯 等一系列生理活性物质 -亚麻酸在心脑血管系统 呼吸系统 肝脏 糖尿性精神 病变和生殖系统具有非常重要的调节作用 利用微生物发酵生产 -亚麻酸已成为国 内外研究的热点 改变培养条件 菌丝体呈现不同的形态特征 分散的微球形和凝聚的球体 研 究发现 分散的微球形形态有利于发酵 生物量和 -亚麻酸产量均较凝聚的球体形 态高 初始pH溶氧量 温度和接种量对菌丝体形态均有显著影响 因此 菌丝体 形态可作为一个直观指标对发酵过程进行控制和了解 通过对细胞生长 油脂积累和 -亚麻酸合成以及营养物质消耗的动力学分析 表明在pH在5.06.0温度23适合发酵 溶氧水平低是造成 -亚麻酸产量低的 原因 建立了描述小克银汉菌培养过程的动力学模型 模型的计算结果和试验测定值 吻合较好 基本能反映培养条件下的过程变化规律 用于培养过程的预测 关键词 小克银汉菌 -亚麻酸 动力学 结构模型 发酵 I Abstract Gamma-linolenic acid (GLA) is a membe of polyunsaturated fatty acids(PUGAs), it can be transformed to PGs and LTs in human bodies,which exert important regulation function in the cardiac and brain blood vessel system,breath system,liver,diabetic neuropathy and genital system. GLA production through microbial fermentation is the hosspot in recent years. Changed the culture contrations, there were different mycelial morphology ,dispersived tiny roudness and agglomerate dollops.The results showed: dispersived tiny roudness was more propitious than agglomerate dollops.pH,dissolved oxygen,temperture and inoculation were effecet on mycelial morphology.So,controlling fermention might be through the mycelial morphology. The time course of consumption of main nutrition composition,fungal biomass,lipid and gamma-linolenic acid accumulation were investigated on a shaker flask scale.Results showed: the optimal culture contration was pH5.0~6.0,temperature 23 .Low dissolved oxygen was resulted to low GLA production. A structural kinetic model was onstructed in this thesis.Calculated result coincided well with experimental data.The model could be used for goood descripition of culture process. Keywords: cunninghamella ; gamma-linolenic; acid; kinetics; structural model; fermentation