benzyl isothiocyanate, a major component from the roots of salvadora persica is highly active against gram-negative bacteria异硫氰酸苄酯,主要组件的根源salvadora persica高度活跃的反对革兰氏阴性细菌.pdfVIP

Benzyl Isothiocyanate, a Major Component from the Roots of Salvadora Persica Is Highly Active against Gram- Negative Bacteria 1 2 2 2 Abier Sofrata , Ellen M. Santangelo , Muhammad Azeem , Anna-Karin Borg-Karlson , Anders 1 ¨ 3 Gustafsson , Katrin Putsep * 1 Department of Dental Medicine, Karolinska Institutet, Stockholm, Sweden, 2 Department of Chemistry, School of Chemical Science and Engineering, Royal Institute of Technology, Stockholm, Sweden, 3 Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden Abstract Plants produce a number of antimicrobial substances and the roots of the shrub Salvadora persica have been demonstrated to possess antimicrobial activity. Sticks from the roots of S. persica, Miswak sticks, have been used for centuries as a traditional method of cleaning teeth. Diverging reports on the chemical nature and antimicrobial repertoire of the chewing sticks from S. persica led us to explore its antibacterial properties against a panel of pathogenic or commensal bacteria and to identify the antibacterial component/s by methodical chemical characterization. S. persica root essential oil was prepared by steam distillation and solid-phase microextraction was used to sample volatiles released from fresh root. The active compound was identified by gas chromatography-mass spectrometry and antibacterial assays. The antibacterial compound was isolated using medium-pressure liquid chromatography. Transmission electron microscopy was used to visualize the effect on bacterial cells. The main antibacterial component of both S. persica root extract