bicaudal d2, dynein, and kinesin-1 associate with nuclear pore complexes and regulate centrosome and nuclear positioning during mitotic entrybicaudal d2、动力蛋白和kinesin-1与核孔复合物和规范中心体和核定位在有丝分裂期间条目.pdfVIP

Bicaudal D2, Dynein, and Kinesin-1 Associate with Nuclear Pore Complexes and Regulate Centrosome and Nuclear Positioning during Mitotic Entry ¨ 1. 2. 2 3 4 1 Daniel Splinter , Marvin E. Tanenbaum , Arne Lindqvist , Dick Jaarsma , Annette Flotho , Ka Lou Yu , 1 5 3 3 6 Ilya Grigoriev , Dieuwke Engelsma , Elize D. Haasdijk , Nanda Keijzer , Jeroen Demmers , Maarten 5 4 3 ´ 2 1 Fornerod , Frauke Melchior , Casper C. Hoogenraad , Rene H. Medema *, Anna Akhmanova * 1 Department of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands, 2 Department of Medical Oncology and Cancer Genomics Center, University Medical Center, Utrecht, The Netherlands, 3 Department of Neuroscience, Erasmus Medical Center, Rotterdam, The Netherlands, 4 Center for Molecular Biology Heidelberg (ZMBH), Heidelberg, Germany, 5 Division of Gene Regulation, The Netherlands Cancer Institute, Amsterdam, The Netherlands, 6 Proteomics Center, Erasmus Medical Center, Rotterdam, The Netherlands Abstract BICD2 is one of the two mammalian homologues of the Drosophila Bicaudal D, an evolutionarily conserved adaptor between microtubule motors and their cargo that was previously shown to link vesicles and mRNP complexes to the dynein motor. Here, we identified a G2-specific role for BICD2 in the relative positioning of the nucleus and centrosomes in dividing cells. By combining mass spectrometry, biochemical and cell biological approaches,