bone marrow cells in murine colitis multi-signal analysis confirms pericryptal myofibroblast engraftment without epithelial involvement骨髓细胞在小鼠结肠炎多信号分析证实pericryptal myofibroblast没有参与上皮移植.pdfVIP

Bone Marrow Cells in Murine Colitis: Multi-Signal Analysis Confirms Pericryptal Myofibroblast Engraftment without Epithelial Involvement 1 1 2 3 1,3 Chung-Yin Lee , Rosemary Jeffery , Gillian Hutchinson , Malcolm R. Alison , Richard Poulsom , Nicholas A. Wright1,3, William R. Otto1,3* 1 Histopathology Laboratory, Cancer Research UK, London Research Institute, London, United Kingdom, 2 Biological Resources Unit, Cancer Research UK, London Research Institute, London, United Kingdom, 3 Centre for Digestive Diseases, Blizard Institute, Barts and The London School of Medicine and Dentistry, London, United Kingdom Abstract Background: The contribution of bone marrow-derived cells to epithelial tissues in the inflamed gut remains controversial. Recent reports have suggested that cell fusion between bone marrow-derived cells and the intestinal epithelium takes place in inflammatory conditions. Methods: In attempts to confirm this, we have undertaken gender mis-matched bone marrow (BM) transplants from male Swiss Webster (SWR) mice to B and T cell-deficient female Rag2 KO mice which, 4 weeks later, were given 5% dextran sodium sulphate in drinking water to induce acute colitis. A further BM-treated group of animals with a graft versus host-like condition was also studied. We developed a new method to combine up to three brightfield or fluorescent lectin- or immuno-histochemical signals with fluorescent in situ hybridisation for the Y and X chromosomes to enable us unequivocally to identify BM-derived male cells which presented as different cell types in the gastrointestinal tract. Principal Findings: In rolled prepara