capsular serotype and antibiotic resistance of streptococcus pneumoniae isolates in malaysia荚膜血清型和抗生素耐药性肺炎链球菌分离株在马来西亚.pdfVIP

Capsular Serotype and Antibiotic Resistance of Streptococcus pneumoniae Isolates in Malaysia 1 2 1 1 Cheng-Foh Le , Navindra Kumari Palanisamy , Mohd Yasim Mohd Yusof , Shamala Devi Sekaran * 1 Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia, 2 Faculty of Medicine, Universiti Teknologi MARA, Shah Alam, Selangor Abstract Background: Streptococcus pneumoniae is a major causative agent of severe infections, including sepsis, pneumonia, meningitis, and otitis media, that has since become a major public health concern. In this study, the serotypes distribution of pneumococcal isolates was investigated to predict the efficacy of the 7-valent pneumococcal conjugate vaccine (PCV7) among the Malaysian populations. Methodology/Principal Findings: A total of 151 clinical isolates were serotyped using multiplex PCR assays. Out of them, there were 21.2% penicillin-resistant, 29.1% penicillin-intermediate, and 49.7% penicillin-susceptible S.pneumoniae strains. Serotypes detected among the Malaysian isolates were 1, 3, 10A, 11A/11D, 12F/12A, 14, 15A, 15B/15C, 16F, 18C/18B/18A/ 18F, 19A, 19F, 23F, 35B, 35F/47F, 6A/6B, 7C/7B/40, 7F/7A, 9V/9A, and 34. Serotype 19F and 23F were the two most prevalent serotypes detected. Serotypes are highly associated with invasiveness of isolates (p = 0.001) and penicillin susceptibility (p,0.001). Serotype 19F was observed to have increased resistance against penicillin while serotype 19A has high invasive tendency. Age of patients was an important factor underlying the pneumococcal serotypes (p = 0.03) and clinical sites of infections (p,0.001). High prevalence of pneumococcal isolates were detecte