brahma is required for proper expression of the floral repressor flc in arabidopsis梵天花卉阻遏的需要适当的表达方法在拟南芥.pdfVIP

Brahma Is Required for Proper Expression of the Floral Repressor FLC in Arabidopsis 1 . 2. ´ 2 3 4 Sara Farrona * , Lidia Hurtado , Rosana March-Dıaz , Robert J. Schmitz , Francisco J. Florencio , 1 3 ´ 2 Franziska Turck , Richard M. Amasino , Jose C. Reyes ´ 1 Max Planck Institute for Plant Breeding, Cologne, Germany, 2 Centro Andaluz de Biologıa Molecular y Medicina Regenerativa (CABIMER), Consejo Superior de ´ Investigaciones Cientıficas (CSIC), Seville, Spain, 3 Department of Biochemistry, University of Wisconsin, Madison, Wisconsin, United States of America, 4 Instituto de ´ ´ ´ Bioquımica Vegetal y Fotosıntesis, Consejo Superior de Investigaciones Cientıficas (CSIC)-Universidad de Sevilla, Seville, Spain Abstract Background: BRAHMA (BRM) is a member of a family of ATPases of the SWI/SNF chromatin remodeling complexes from Arabidopsis. BRM has been previously shown to be crucial for vegetative and reproductive development. Methodology/Principal Findings: Here we carry out a detailed analysis of the flowering phenotype of brm mutant plants which reveals that, in addition to repressing the flowering promoting genes CONSTANS (CO), FLOWERING LOCUS T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CO1 (SOC1), BRM also represses expression of the general flowering repressor FLOWERING LOCUS C (FLC). Thus, in brm mutant plants FLC expression is elevated, and FLC chromatin exhibits increased