comparative analysis of dynamic cell viability, migration and invasion assessments by novel real-time technology and classic endpoint assays比较分析动态细胞生存能力、迁移和入侵评估通过新颖的实时技术和经典的端点检测.pdfVIP

Comparative Analysis of Dynamic Cell Viability, Migration and Invasion Assessments by Novel Real-Time Technology and Classic Endpoint Assays 1 1 1 2 1,3 1 Ridha Limame *, An Wouters , Bea Pauwels , Erik Fransen , Marc Peeters , Filip Lardon , Olivier De Wever4, Patrick Pauwels1,5 1 Center for Oncological Research (CORE), University of Antwerp, Antwerp, Belgium, 2 StatUA Center for Statistics, University of Antwerp, Antwerp, Belgium, 3 Department of Oncology, Antwerp University Hospital, Edegem (Antwerp), Belgium, 4 Laboratory of Experimental Cancer Research, Department of Radiotherapy and Nuclear Medicine, Ghent University Hospital, Ghent, Belgium, 5 Laboratory of Pathology, Antwerp University Hospital, Edegem (Antwerp), Belgium Abstract Background: Cell viability and motility comprise ubiquitous mechanisms involved in a variety of (patho)biological processes including cancer. We report a technical comparative analysis of the novel impedance-based xCELLigence Real-Time Cell Analysis detection platform, with conventional label-based endpoint methods, hereby indicating performance characteristics and correlating dynamic observations of cell proliferation, cytotoxicity, migration and invasion on cancer cells in highly standardized experimental conditions. Methodology/Principal Findings: Dynamic high-resolution assessments of proliferation, cytotoxicity and migration were performed using xCELLigence technology on the MDA-MB-231 (breast cancer) and A549 (lung cancer) cell lines. Proliferation kinetics were compared with the Sulforhodamine B (SRB) assay in a series of four cell concentrations, yielding fair to good correla