comparative expression profiling of the chlamydia trachomatis pmp gene family for clinical and reference strains比较表达分析沙眼衣原体的pmp临床和参考菌株的基因家族.pdfVIP

Comparative Expression Profiling of the Chlamydia trachomatis pmp Gene Family for Clinical and Reference Strains 1,2 ˜ 1,2 1 2 2 2 1,3,4 Alexandra Nunes , Joao P. Gomes , Sally Mead , Carlos Florindo , Helena Correia , Maria J. Borrego , Deborah Dean * 1 Center for Immunobiology and Vaccine Development, Children’s Hospital Oakland Research Institute, Oakland, California, United States of America, ´ 2 Departamento de Bacteriologia, Instituto Nacional de Saude, Lisboa, Portugal, 3 Department of Bioengineering, University of California at Berkeley, Berkeley, California, United States of America, 4 Department of Medicine, School of Medicine, University of California at San Francisco, San Francisco, California, United States of America Background. Chlamydia trachomatis, an obligate intracellular pathogen, is a leading worldwide cause of ocular and urogenital diseases. Advances have been made in our understanding of the nine-member polymorphic membrane protein (Pmp) gene (pmp) family of C. trachomatis. However, there is only limited information on their biologic role, especially for biological variants (biovar) and clinical strains. Methodology/Principal Findings. We evaluated expression for pmps throughout development for reference strains E/Bour and L2/434, representing different biovars, and for clinical E and L2 strains. Immunoreactivity of patient sera to recombinant (r)Pmps was also determined. All pmps were expressed at two hours. pmpA had the lowest expression but was up-regulated at 12 h for all strains, indicating involvement in reticulate body development. For pmpD, expression peaked at 36 h. Additionally, 57.7%