cigarette smoking blocks the protective expression of nrf2are pathway in peripheral mononuclear cells of young heavy smokers favouring inflammation吸烟街区的保护表达nrf2are通路在周边单核细胞年轻的重度吸烟者支持炎症.pdfVIP

Cigarette Smoking Blocks the Protective Expression of Nrf2/ARE Pathway in Peripheral Mononuclear Cells of Young Heavy Smokers Favouring Inflammation 1 1 1 1 1 Ulisse Garbin , Anna Fratta Pasini , Chiara Stranieri , Mattia Cominacini , Andrea Pasini , Stefania 1 2 1 3 3 1 Manfro , Fabio Lugoboni , Chiara Mozzini , GianCesare Guidi , Giovanni Faccini , Luciano Cominacini * 1 Department of Biomedical and Surgical Sciences, Section of Internal Medicine, University of Verona, Verona, Italy, 2 Medical Service for Addictive Disorders, University Hospital G. B. Rossi, Verona, Italy, 3 Department of Morphological and Biomedical Sciences, Section of Chemistry and Clinical Microscopy, University of Verona, Verona, Italy Abstract Cigarette smoking is an important risk factor for atherosclerosis, a chronic inflammatory disease. However the underlying factors of this effect are unclear. It has been hypothesized that water-soluble components of cigarette smoke can directly promote oxidative stress in vasculature and blood cells. Aim of this study was to study the relationship between oxidative stress and inflammation in a group of young smokers. To do this we evaluated: 1) the oxidation products of phospholipids (oxPAPC) in peripheral blood mononuclear cells (PBMC); 2) their role in causing PBMC reactive oxygen species (ROS) generation and changes in GSH; 3) the expression of the transcription factor NF-E2-related factor 2 (Nrf2) and of related antioxidant genes (ARE); 4) the activation of NF-kB and C-reactive protein (CRP) values. We studied 90 healthy volunteers: 32 non-smokers, 32 moderate smokers (5–10 cig