changes in global gene expression in response to chemical and genetic perturbation of chromatin structure全球基因表达的变化反应化学和基因染色质结构的扰动.pdfVIP

Changes in Global Gene Expression in Response to Chemical and Genetic Perturbation of Chromatin Structure Karen Hudson¤a, Song Luo¤b, Nicole Hagemann, Daphne Preuss*¤b Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois, United States of America Abstract DNA methylation is important for controlling gene expression in all eukaryotes. Microarray analysis of mutant and chemically- treated Arabidopsis thaliana seedlings with reduced DNA methylation revealed an altered gene expression profile after treatment with the DNA methylation inhibitor 5-aza-29 deoxycytidine (5-AC), which included the upregulation of expression of many transposable elements. DNA damage-response genes were also coordinately upregulated by 5-AC treatment. In the ddm1 mutant, more specific changes in gene expression were observed, in particular for genes predicted to encode transposable elements in centromeric and pericentromeric locations. These results confirm that DDM1 has a very specific role in maintaining transcriptional silence of transposable elements, while chemical inhibitors of DNA methylation can affect gene expression at a global level. Citation: Hudson K, Luo S, Hagemann N, Preuss D (2011) Changes in Global Gene Expression in Response to Chemical and Genetic Perturbation of Chromatin Structure. PLoS ONE 6(6): e20587. doi:10.1371/journal.pone.0020587 ¨ ¨ Editor: Axel Imhof, Ludwig-Maximilians-Universitat Munchen, Germany Received November 3, 2010; Accepted May 6, 2011; Published June 3, 2011 This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. Funding: The