comparative effects of heterologous trpv1 and trpm8 expression in rat hippocampal neurons比较的影响不同的trpv1和trpm8表达在鼠海马神经元.pdfVIP

Comparative Effects of Heterologous TRPV1 and TRPM8 Expression in Rat Hippocampal Neurons 1,2 2 3,4 3,4 2,4 Devon C. Crawford , Krista L. Moulder , Robert W. Gereau, IV , Gina M. Story , Steven Mennerick * 1 Graduate Program in Neurosciences, Washington University, St. Louis, Missouri, United States of America, 2 Department of Psychiatry, Washington University, St. Louis, Missouri, United States of America, 3 Department of Anesthesiology, Washington University, St. Louis, Missouri, United States of America, 4 Department of Anatomy and Neurobiology, Washington University, St. Louis, Missouri, United States of America Abstract Heterologous channel expression can be used to control activity in select neuronal populations, thus expanding the tools available to modern neuroscience. However, the secondary effects of exogenous channel expression are often left unexplored. We expressed two transient receptor potential (TRP) channel family members, TRPV1 and TRPM8, in cultured hippocampal neurons. We compared functional expression levels and secondary effects of channel expression and activation on neuronal survival and signaling. We found that activation of both channels with appropriate agonist caused large depolarizing currents in voltage-clamped hippocampal neurons, exceeding the amplitude responses to a calibrating 30 mM KCl stimulation. Both TRPV1 and TRPM8 currents were reduced but not eliminated by 4 hr incubation in saturating agonist concentration. In the case of TRPV1, but not TRPM8, prolonged agonist exposure caused strong calcium-