comparative genomics of cell envelope components in mycobacteria比较基因组学在分枝杆菌细胞被膜组件.pdfVIP

Comparative Genomics of Cell Envelope Components in Mycobacteria . . . Ruma Banerjee , Pankaj Vats , Sonal Dahale , Sunitha Manjari Kasibhatla, Rajendra Joshi* Bioinformatics Group, Centre for Development of Advanced Computing, Pune University Campus, Pune, Maharashtra, India Abstract Mycobacterial cell envelope components have been a major focus of research due to their unique features that confer intrinsic resistance to antibiotics and chemicals apart from serving as a low-permeability barrier. The complex lipids secreted by Mycobacteria are known to evoke/repress host-immune response and thus contribute to its pathogenicity. This study focuses on the comparative genomics of the biosynthetic machinery of cell wall components across 21-mycobacterial genomes available in GenBank release 179.0. An insight into survival in varied environments could be attributed to its variation in the biosynthetic machinery. Gene-specific motifs like ‘DLLAQPTPAW’ of ufaA1 gene, novel functional linkages such as involvement of Rv0227c in mycolate biosynthesis; Rv2613c in LAM biosynthesis and Rv1209 in arabinogalactan peptidoglycan biosynthesis were detected in this study. These predictions correlate well with the available mutant and coexpression data from TBDB. It also helped to arrive at a minimal functional gene set for these biosynthetic pathways that complements findings using TraSH. Citation: Banerjee R, Vats P, Dahale S, Kasibhatla SM, Joshi R (2011) Comparative Genomics of Cell Envelope Components in Mycobacteria. PLoS ONE 6(5): e19280. doi:10.1371/journal.pone.0019280 Editor: Olivier Neyrolles, Institut de Pharmacologie et de Biologie Structurale, France Received January 27, 2011; Accepted March 25, 2011; Published