circulating antinuclear antibodies in patients with pelvic masses are associated with malignancy and decreased survival盆腔肿块患者循环抗核抗体与恶性肿瘤和减少生存.pdfVIP

Circulating Antinuclear Antibodies in Patients with Pelvic Masses Are Associated with Malignancy and Decreased Survival 1 1 1 1 2 Niels H. H. Heegaard *, Mikkel West-Nørager , Julia T. Tanassi , Gunnar Houen , Lotte Nedergaard , Claus Høgdall3, Estrid Høgdall4 1 Department of Clinical Biochemistry and Immunology, Statens Serum Institut, Copenhagen, Denmark, 2 Department of Pathology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark, 3 The Gynecological Clinic, The Juliane Marie Center, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark, 4 Department of Pathology, Herlev Hospital, University of Copenhagen, Herlev, Denmark Abstract Background: Circulating autoantibodies occur more frequently in cancer patients than in patients without cancer. Methods and Findings: We examined sera from patients referred for pelvic mass symptoms to a tertiary university clinic. A total of 127 were diagnosed with epithelial ovarian cancer while 386 had a benign condition. A screen for IgG anti-nuclear antibodies (ANA) by indirect immunofluorescence on HEp-2 cells confirmed a highly significant overrepresentation of ANA in the cancer group where 40% had detectable (i.e., a titer $160) ANA compared with less than 12% in the benign group. The overrepresentation of ANA in the cancer group persisted (p ,0.0001) after matching the age-profile of the benign group with the ovarian cancer group. Only 19 out of 127 patients in the age-matched benign subgroup were positive for ANA corresponding to an 85% specificity at 40% sensitivity of ANA as the only marker for malignancy. No correlation of ANA positivity in either group with specific bands in immunoblots could be demonstrated even though immunoblot positivity was clearly increased in t