diagnostic accuracy of molecular amplification tests for human african trypanosomiasis—systematic review分子诊断的准确性为非洲人类trypanosomiasis-systematic放大测试评估.pdfVIP

Diagnostic Accuracy of Molecular Amplification Tests for Human African Trypanosomiasis—Systematic Review 1,2. 1 . 1,3 4 ¨ 5 Claire M. Mugasa , Emily R. Adams * , Kimberly R. Boer , Heleen C. Dyserinck , Philippe Buscher , Henk D. H. F. Schallig1, Mariska M. G. Leeflang6 1 Royal Tropical Institute, KIT Biomedical Research, Amsterdam, The Netherlands, 2 Department of Veterinary Parasitology and Microbiology, Faculty of Veterinary Medicine, Makerere University Kampala, Kampala, Uganda, 3 Amsterdam Institute of Global Health and Development, Amsterdam, The Netherlands, 4 Academic Medical Centre Library, Academic Medical Centre, Amsterdam, The Netherlands, 5 Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium, 6 Department of Clinical Epidemiology, Biostatistics and Bioinformatics, Academic Medical Centre, Amsterdam, The Netherlands Abstract Background: A range of molecular amplification techniques have been developed for the diagnosis of Human African Trypanosomiasis (HAT); however, careful evaluation of these tests must precede implementation to ensure their high clinical accuracy. Here, we investigated the diagnostic accuracy of molecular amplification tests for HAT, the quality of articles and reasons for variation in accuracy. Methodology: Data from studies assessing diagnostic molecular amplification tests were extracted and pooled to calculate accuracy. Articles were included if they reported sensitivity and specificity or data whereby values could be calculated. Study quality was assessed using QUADAS and selected studies were analysed using the bivariate random effects model. Results: 16 articles evaluating molecular amplification te