effect of correlated trna abundances on translation errors and evolution of codon usage bias相关trna丰度对翻译错误和演化的影响密码子使用的偏见.pdfVIP

Effect of Correlated tRNA Abundances on Translation Errors and Evolution of Codon Usage Bias Premal Shah1,2*, Michael A. Gilchrist1,2 1 Department of Ecology Evolutionary Biology, University of Tennessee, Knoxville, Tennessee, United States of America, 2 National Institute for Mathematical and Biological Synthesis, University of Tennessee, Knoxville, Tennessee, United States of America Abstract Despite the fact that tRNA abundances are thought to play a major role in determining translation error rates, their distribution across the genetic code and the resulting implications have received little attention. In general, studies of codon usage bias (CUB) assume that codons with higher tRNA abundance have lower missense error rates. Using a model of protein translation based on tRNA competition and intra-ribosomal kinetics, we show that this assumption can be violated when tRNA abundances are positively correlated across the genetic code. Examining the distribution of tRNA abundances across 73 bacterial genomes from 20 different genera, we find a consistent positive correlation between tRNA abundances across the genetic code. This work challenges one of the fundamental assumptions made in over 30 years of research on CUB that codons with higher tRNA abundances have lower missense error rates and that missense errors are the primary selective force responsible for CUB. Citation: Shah P, Gilchrist MA (2010) Effect of Correlated tRNA Abundances on Translation Errors and Evolution of Codon Usage Bias. PLoS Genet 6(9): e1001128. doi:10.1371/journal.pgen.1001128 Editor: Allan Drummond, Harvard University, United States of America Received April 19, 2010; Accepted August 18, 2010; Published September 16, 2010 Copyright: 2010 Shah, Gilchrist. This is an open-access article distributed under the terms of the Creative Common