effect of dlk1 and rtl1 but not meg3 or meg8 on muscle gene expression in callipyge lambsdlk1和rtl1但不是meg3或meg8 callipyge羊羔的肌肉基因表达.pdfVIP

Effect of DLK1 and RTL1 but Not MEG3 or MEG8 on Muscle Gene Expression in Callipyge Lambs 1 2 1,3 4 5 Jolena N. Fleming-Waddell , Gayla R. Olbricht , Tasia M. Taxis , Jason D. White , Tony Vuocolo , 2 5 1 6 1 Bruce A. Craig , Ross L. Tellam , Mike K. Neary , Noelle E. Cockett , Christopher A. Bidwell * 1 Department of Animal Sciences, Purdue University, West Lafayette, Indiana, United States of America, 2 Department of Statistics, Purdue University, West Lafayette, Indiana, United States of America, 3 Animal Sciences Division, University of Missouri, Columbia, Missouri, United States of America, 4 School of Veterinary Science, The University of Melbourne, Parkville, Victoria, Australia, 5 CSIRO Livestock Industries, St. Lucia, Queensland, Australia, 6 Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, Utah, United States of America Abstract Callipyge sheep exhibit extreme postnatal muscle hypertrophy in the loin and hindquarters as a result of a single nucleotide polymorphism (SNP) in the imprinted DLK1-DIO3 domain on ovine chromosome 18. The callipyge SNP up-regulates the expression of surrounding transcripts when inherited in cis without altering their allele-specific imprinting status. The callipyge phenotype exhibits polar overdominant inheritance since only paternal heterozygous animals have muscle hypertrophy. Two studies were conducted profiling gene expression in lamb muscles to determine the down-stream effects of over-expression of paternal allele-specific DLK1 and RTL1 as well as maternal