effect of fibrin glue on the biomechanical properties of human descemets membrane纤维蛋白胶对人体的生物力学特性的影响角膜后弹力层.pdfVIP

Effect of Fibrin Glue on the Biomechanical Properties of Human Descemet’s Membrane 1 1 2 1 1 Shyam S. Chaurasia , Ravi Champakalakshmi , Ang Li , Rebekah Poh , Xiao Wei Tan , Rajamani Lakshminarayanan1, Chwee T. Lim2,3, Donald T. Tan1,4, Jodhbir S. Mehta1,4,5,6* 1Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore, Singapore, 2 Department of Bioengineering and Department of Mechanical Engineering, National University of Singapore, Singapore, Singapore, 3 Mechanobiology Institute, Singapore, Singapore, 4 Singapore National Eye Centre, Singapore, Singapore, 5 Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore, 6 Department of Clinical Sciences, Duke-NUS Graduate Medical School, Singapore, Singapore Abstract Background: Corneal transplantation has rapidly evolved from full-thickness penetrating keratoplasty (PK) to selective tissue corneal transplantation, where only the diseased portions of the patient’s corneal tissue are replaced with healthy donor tissue. Descemet’s membrane endothelial keratoplasty (DMEK) performed in patients with corneal endothelial dysfunction is one such example where only a single layer of endothelial cells with its basement membrane (10–15 mm in thickness), Descemet’s membrane (DM) is replaced. It is challenging to replace this membrane due to its intrinsic property to roll in an aqueous environment. The main objective of this study was to determine the effects of fibrin glue (FG) on the biomechanical properties of DM using atomic force microscopy (AFM) and relates these properties to membrane folding propensity. Methodology/Principal Findings: Fibrin glue was sprayed