efficient activation of reconstructed rat embryos by cyclin-dependent kinase inhibitors有效激活重建大鼠胚胎的细胞周期蛋白依赖性激酶抑制剂.pdfVIP

Efficient Activation of Reconstructed Rat Embryos by Cyclin-Dependent Kinase Inhibitors 1,2 3 4 2 1,2 Robin L. Webb , Kirk A. Findlay , Michael A. Green , Tina L. Beckett , M. Paul Murphy * 1 Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, Kentucky, United States of America, 2 Sanders-Brown Center on Aging, University of Kentucky, Lexington, Kentucky, United States of America, 3 Department of Neuroscience, Mayo Clinic Jacksonville, Jacksonville, Florida, United States of America, 4 Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, Kentucky, United States of America Abstract Background: Over the last decade a number of species, from farm animals to rodents, have been cloned using somatic cell nuclear transfer technology (SCNT). This technique has the potential to revolutionize the way that genetically modified animals are made. In its current state, the process of SCNT is very inefficient (,5% success rate), with several technical and biological hurdles hindering development. Yet, SCNT provides investigators with powerful advantages over other approaches, such as allowing for prescreening for the desired level of transgene expression and eliminating the excess production of undesirable wild-type animals. The rat plays a significant role in biomedical research, but SCNT has been problematic for this species. In this study, we address one aspect of the problem by evaluating methods of activation in artificially constructed rat embryos. Principal Findings: We demonstrate that treatment with a calcium ionophore (ionom