efficient removal of platelets from peripheral blood progenitor cell products using a novel micro-chip based acoustophoretic platform有效的去除血小板从外周血祖细胞产品使用基于一种新型芯片acoustophoretic平台.pdfVIP

Efficient Removal of Platelets from Peripheral Blood Progenitor Cell Products Using a Novel Micro-Chip Based Acoustophoretic Platform 1,2 3 ˚ ¨ 2 3,5 Josefina Dykes *, Andreas Lenshof , Ing-Britt Astrand-Grundstrom , Thomas Laurell , Stefan Scheding2,4 1 Clinical Immunology and Transfusion Medicine, Regional and University Laboratories, Lund, Sweden, 2 Stem Cell Center, Institution of Laboratory Medicine, Lund University, Lund, Sweden, 3 Measurement Technology and Industrial Electrical Engineering, Lund University, Lund, Sweden, 4 Department of Hematology, University Hospital Skane, Lund, Sweden, 5 Biomedical Engineering, Dongguk University, Seoul, Korea Abstract Background: Excessive collection of platelets is an unwanted side effect in current centrifugation-based peripheral blood progenitor cell (PBPC) apheresis. We investigated a novel microchip-based acoustophoresis technique, utilizing ultrasonic standing wave forces for the removal of platelets from PBPC products. By applying an acoustic standing wave field onto a continuously flowing cell suspension in a micro channel, cells can be separated from the surrounding media depending on their physical properties. Study Design and Methods: PBPC samples were obtained from patients (n = 15) and healthy donors (n = 6) and sorted on an acoustophoresis-chip. The acoustic force was set to separate leukocytes from platelets into a target fraction and a waste fraction, respectively. The PBPC samples, the target and the waste fractions were analysed for cell recovery, purity and functionality. Results: The median separation efficiency of leukocytes to the target fraction was 98% whereas platelets were effectively