genotype-temperature interaction in the regulation of development, growth, and morphometrics in wild-type, and growth-hormone transgenic coho salmongenotype-temperature互动发展的规定,增长,在野生型的形态学、生长激素转基因银鲑鱼.pdfVIP

Genotype-Temperature Interaction in the Regulation of Development, Growth, and Morphometrics in Wild-Type, and Growth-Hormone Transgenic Coho Salmon ˜ 1,2 ¨ 1 ¨ 3 1 Mare Lohmus *, L. Fredrik Sundstrom , Mats Bjorklund , Robert H. Devlin 1 Centre for Aquaculture and Environmental Research, Fisheries and Oceans Canada, West Vancouver, British Columbia, Canada, 2 Section for Environment and Biosecurity, Department for Chemistry, Environment and Feed Hygiene, National Veterinary Institute, Uppsala, Sweden, 3 Animal Ecology/Department of Ecology and Evolution, Evolutionary Biology Centre, Uppsala University, Uppsala, Sweden Abstract Background: The neuroendocrine system is an important modulator of phenotype, directing cellular genetic responses to external cues such as temperature. Behavioural and physiological processes in poikilothermic organisms (e.g. most fishes), are particularly influenced by surrounding temperatures. Methodology/Principal Findings: By comparing the development and growth of two genotypes of coho salmon (wild-type and transgenic with greatly enhanced growth hormone production) at six different temperatures, ranging between 8u and 18uC, we observed a genotype-temperature interaction and possible trend in directed neuroendocrine selection. Differences in growth patterns of the two genotypes were compared by using mathematical models, and morphometric analyses of juvenile salmon were performed to detect differences in body shape. The maximum hatching and alevin survival rates of both genotypes occurred at 12uC. At lower temperatures, eggs containing embryos with enhanced GH production hatched after a shorter incubation period than wild-type eggs, but this difference was not apparent at and above 16uC. GH