no detection of xmrv in blood samples and tissue sections from prostate cancer patients in northern europe没有发现xmrv血样和组织部分从欧洲北部的前列腺癌患者.pdfVIP

No Detection of XMRV in Blood Samples and Tissue Sections from Prostate Cancer Patients in Northern Europe 1 1,2 3 4 4 5 Kristin Stieler , Sarah Schindler , Thorsten Schlomm , Oliver Hohn , Norbert Bannert , Ronald Simon , 5 2 1 Sarah Minner , Michael Schindler , Nicole Fischer * 1 Institute for Medical Microbiology and Virology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany, 2 Heinrich-Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany, 3 Martini-Clinic, Prostate Cancer Center, University Medical Center Hamburg-Eppendorf, Hamburg, Germany, 4 Robert Koch Institute, Center for HIV and Retrovirology, Berlin, Germany, 5 Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany Abstract Background: We recently published the rare detection of xenotropic murine leukemia virus-related virus (XMRV) (1/105) in prostate cancer (PCA) tissue of patients in Northern Europe by PCR. The controversial discussion about the virus being detected in PCA tissue, blood samples from patients suffering from chronic fatigue syndrome (CFS), as well as from a significant number of healthy controls prompted us to deepen our studies about detection of XMRV infection applying different detection methods (PCR, cocultivation and immunohistochemistry [IHC]). Methodology/Principal Findings: Peripheral blood mononuclear cells (PBMCs) from 92 PCA and 7 healthy controls were isolated, PHA activated and cocultivated with LNCaP cells for up to 8 weeks. Supernatant of these cells was applied to a reporter cell line, DERSE-iGFP. Furthermore, the PBMCs and cocultivated LNCaP c