no gold standard estimation of the sensitivity and specificity of two molecular diagnostic protocols for trypanosoma brucei spp. in western kenya没有黄金标准估计两个分子的敏感性和特异性诊断协议锥虫属brucei种虫害在肯尼亚西部.pdfVIP

No Gold Standard Estimation of the Sensitivity and Specificity of Two Molecular Diagnostic Protocols for Trypanosoma brucei spp . in Western Kenya 3 1 ` 2 3 Barend Mark de Clare Bronsvoort *, Beatrix von Wissmann , Eric Maurice Fevre , Ian Graham Handel , Kim Picozzi1, Sue Christina Welburn1 1 Centre for Infectious Diseases, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United Kingdom, 2 Centre for Infectious Diseases and Centre for Infection, Immunity and Evolution, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom, 3 The Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian, United Kingdom Abstract African animal trypanosomiasis is caused by a range of tsetse transmitted protozoan parasites includingTrypanosoma vivax, Trypanosoma congolense and Trypansoma brucei. In Western Kenya and other parts of East Africa two subspecies of T. brucei, T.b. brucei and the zoonoticT.b. rhodesiense, co-circulate in livestock. A range of polymerase chain reactions (PCR) have been developed as important molecular diagnostic tools for epidemiological investigations of T. brucei s.l. in the animal reservoir and of its zoonotic potential. Quantification of the relative performance of different diagnostic PCRs is essential to ensure comparability of studies. This paper describes an evaluation of two diagnostic test systems for T. brucei using a T. brucei s.l. specific PCR [1] and a single nested PCR targeting the Internal Transcribed Spacer (ITS) regions of trypanosome ribosomal DNA [2]. A Bayesian formulation of the Hui-Walter latent class model was employed to estimate their test performance in the absen