noise-induced min phenotypes in e. coli噪音性敏在大肠杆菌表型.pdfVIP

Noise-Induced Min Phenotypes in E. coli 1 1,2* David Fange , Johan Elf 1 Department of Cell and Molecular Biology, Biomedical Centre, Uppsala University, Uppsala, Sweden, 2 Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, United States of America The spatiotemporal oscillations of the Escherichia coli proteins MinD and MinE direct cell division to the region between the chromosomes. Several quantitative models of the Min system have been suggested before, but no one of them accounts for the behavior of all documented mutant phenotypes. We analyzed the stochastic reaction-diffusion kinetics of the Min proteins for several E. coli mutants and compared the results to the corresponding deterministic mean-field description. We found that wild-type (wt) and filamentous (ftsZ ) cells are well characterized by the mean- field model, but that a stochastic model is necessary to account for several of the characteristics of the spherical (rodA ) and phospathedylethanolamide-deficient (PE) phenotypes. For spherical cells, the mean-field model is bistable, and the system can get trapped in a non-oscillatory state. However, when the intrinsic noise is considered, only the experimentally observed oscillatory behavior remains. The stochastic model also reproduces the change in oscillation directions observed in the spherical phenotype and the occasional gliding of the MinD region along the inner membrane. For the PE mutant, the stochastic model explains the appearance of randomly localized and dense MinD clusters as a nucleation phe