non-invasive imaging of acute renal allograft rejection in rats using small animal 18f-fdg-pet急性肾移植排斥反应的非侵入性成像使用小动物18 f-fdg-pet老鼠.pdfVIP

Non-Invasive Imaging of Acute Renal Allograft Rejection in Rats Using Small Animal 18F-FDG-PET 1. ¨ 2. ¨ 1 2 ¨ 1 Stefan Reuter *, Uta Schnockel , Rita Schroter , Otmar Schober , Hermann Pavenstadt , Michael ¨ 2 ¨ 1 1 Schafers , Gert Gabriels , Eberhard Schlatter ¨ ¨ ¨ ¨ 1 Medizinische Klinik und Poliklinik D, Experimentelle Nephrologie, Universitatsklinikum Munster, Munster, Germany, 2 Klinik und Poliklinik fur Nuklearmedizin, ¨ ¨ ¨ Universitatsklinikum Munster, Munster, Germany Abstract Background: At present, renal grafts are the most common solid organ transplants world-wide. Given the importance of renal transplantation and the limitation of available donor kidneys, detailed analysis of factors that affect transplant survival are important. Despite the introduction of new and effective immunosuppressive drugs, acute cellular graft rejection (AR) is still a major risk for graft survival. Nowadays, AR can only be definitively by renal biopsy. However, biopsies carry a risk of renal transplant injury and loss. Most important, they can not be performed in patients taking anticoagulant drugs. Methodology/Principal Findings: We present a non-invasive, entirely image-based method to assess AR in an allogeneic rat renal transplantation model using small animal positron emission tomography (PET) and 18F-fluorodeoxyglucose (FDG). 3 h after i.v. injection of 30 MBq FDG into adult uni-nephrectomized, allogeneically transplanted rats, tissue radioactiv