notch2 is neither rearranged nor mutated in t(1;19) positive oligodendrogliomasnotch2既不重新安排也不突变在t(1;19)正少突神经胶质瘤.pdfVIP

NOTCH2 Is Neither Rearranged nor Mutated in t(1;19) Positive Oligodendrogliomas Magdalena Benetkiewicz1,2, Ahmed Idbaih3,4,5, Pierre-Yves Cousin1,2, Blandine Boisselier3,4, Yannick 3,4 ` 3,4 ˆ 3,4,5 3,4,5 3,4,5 Marie , Emmanuelle Criniere , Khe Hoang-Xuan , Jean-Yves Delattre , Marc Sanson , Olivier Delattre1,2* ´ ´ ´ ´ ´ 1 INSERM, Unite 830, Paris, France, 2 Institut Curie, Section Recherche, Unite de Genetique et Biologie des Cancers, Paris, France, 3 INSERM, Unite 711, Groupe hospitalier ´ ˆ ` ´ ´ ˆ ` Pitie-Salpetriere, Paris, France, 4 Universite Pierre et Marie Curie-Paris 6, Laboratoire Biologie des Interactions Neurone-Glie, Groupe hospitalier Pitie-Salpetriere, Paris, ´ ˆ ` France, 5 AP-HP, Groupe hospitalier Pitie-Salpetriere, Service de neurologie Mazarin, Paris, France Abstract The combined deletion of 1p and 19q chromosomal arms is frequent in oligodendrogliomas (OD) and has recently been shown to be mediated by an unbalanced t(1;19) translocation. Recent studies of 1p/19q co-deleted OD suggest that the NOTCH2 gene is implicated in oligodendrocyte differentiation and may be involved in this rearrangement. The objective of the present study was to analyze the NOTCH2 locus either as a chromosomal translocation locus that may be altered by the 1p/19q recurrent rearrangement or as a gene that may be inactivated by a two hit process. We performed an arr