novel biomarkers distinguishing active tuberculosis from latent infection identified by gene expression profile of peripheral blood mononuclear cells新型生物标记区分活动性结核病和潜伏性感染了外周血单核细胞的基因表达谱.pdfVIP

Novel Biomarkers Distinguishing Active Tuberculosis from Latent Infection Identified by Gene Expression Profile of Peripheral Blood Mononuclear Cells 1,2 1,2 2 1 1 1 1 1 Chanyi Lu , Jing Wu , Honghai Wang , Sen Wang , Ni Diao , Feifei Wang , Yan Gao , Jiazhen Chen , 1 1 1,3 1,4 Lingyun Shao , Xinhua Weng , Ying Zhang *, Wenhong Zhang * 1 Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China, 2 State Key Laboratory of Genetic Engineering(SKLGE), Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, China, 3 Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, United States of America, 4 Institutes of Biomedical Sciences, Fudan University, Shanghai, China Abstract Background: Humans infected with Mycobacterium tuberculosis (MTB) can delete the pathogen or otherwise become latent infection or active disease. However, the factors influencing the pathogen clearance and disease progression from latent infection are poorly understood. This study attempted to use a genome-wide transcriptome approach to identify immune factors associated with MTB infection and novel biomarkers that can distinguish active disease from latent infection. Methodology/Principal Findings: Using microarray analysis, we comprehensively determined the transcriptional difference in purified protein derivative (PPD) stimulated peripheral blood mononuclear cells (PBMCs) in 12 individuals divided into three groups: TB patients (TB), latent TB infection individuals (LTBI) and healthy controls (