novel insights into the diversity of catabolic metabolism from ten haloarchaeal genomes新颖的见解从十haloarchaeal分解代谢基因的多样性.pdfVIP

Novel Insights into the Diversity of Catabolic Metabolism from Ten Haloarchaeal Genomes 1 2 ¨ 2 1 1 3 Iain Anderson *, Carmen Scheuner , Markus Goker , Kostas Mavromatis , Sean D. Hooper , Iris Porat , 2 1 1 Hans-Peter Klenk , Natalia Ivanova , Nikos Kyrpides 1 Genome Biology Program, Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America, 2 Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany, 3 INEOS Bio, Fayetteville, Arkansas, United States of America Abstract Background: The extremely halophilic archaea are present worldwide in saline environments and have important biotechnological applications. Ten complete genomes of haloarchaea are now available, providing an opportunity for comparative analysis. Methodology/Principal Findings: We report here the comparative analysis of five newly sequenced haloarchaeal genomes with five previously published ones. Whole genome trees based on protein sequences provide strong support for deep relationships between the ten organisms. Using a soft clustering approach, we identified 887 protein clusters present in all halophiles. Of these core clusters, 112 are not found in any other archaea and therefore constitute the haloarchaeal signature. Four of the halophiles were isolated from water, and four were isolated from soil or sediment. Although there are few habitat-specific clusters, the soil/sediment halophiles tend to have greater capacity for polysaccharide degradation, siderophore synthesis, and cell wall modification. Halorhabdus utahensis and Halot