novel molecular and computational methods improve the accuracy of insertion site analysis in sleeping beauty-induced tumors新颖的分子和计算方法改善睡眠的插入网站分析的准确性beauty-induced肿瘤.pdfVIP

Novel Molecular and Computational Methods Improve the Accuracy of Insertion Site Analysis in Sleeping Beauty-Induced Tumors 1 2 1,3 4,5 Benjamin T. Brett , Katherine E. Berquam-Vrieze , Kishore Nannapaneni , Jian Huang , Todd E. Scheetz1,3,6, Adam J. Dupuy2,7* 1 Center for Bioinformatics and Computational Biology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America, 2 Department of Anatomy and Cell Biology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America, 3 Department of Biomedical Engineering, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America, 4 Department of Statistics and Actuarial Science, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America, 5 Department of Biostatistics, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America, 6 Department of Ophthalmology and Visual Sciences, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America, 7 Department of Pathology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America Abstract The recent development of the Sleeping Beauty (SB) system has led to the development of novel mouse models of cancer. Unlike spontaneous models, SB causes cancer through the action of mutagenic transposons that are mobilized in the genomes of somatic cells to induce mutations in cancer genes. While previous methods have successfully identified many transposon-tagged mutations in