role of apoptotic proteins in rec-2006 mediated radiation protection in hepatoma cell linesrec - 2006年凋亡蛋白介导的作用在肝癌细胞株辐射防护.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 758326, 11 pages doi:10.1093/ecam/neq059 Original Article Role of Apoptotic Proteins in REC-2006 Mediated Radiation Protection in Hepatoma Cell Lines Pankaj Kumar Singh,1 Raj Kumar,1 Ashok Sharma,1 Rajesh Arora,1 Raman Chawla,1 Swatantra Kumar Jain,2 Rajendra Prasad Tripathi,1 and Rakesh Kumar Sharma1 1 Institute of Nuclear Medicine and Allied Sciences, Delhi, India 2 Jamia Hamdard, Hamdard Nagar, Delhi, India Correspondence should be addressed to Rakesh Kumar Sharma, rks@inmas.drdo.in Received 20 December 2009; Accepted 9 April 2010 Copyright © 2011 Pankaj Kumar Singh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The present study was carried out to evaluate the role of apoptotic proteins in REC-2006-mediated radiation protection in hepatoma cell lines. REC-2006 treatment 2 h before irradiation strongly inhibited the cleavage of ATM and PARP-1 in HepG2 cells. The expression of nuclear apoptosis inducing factor (AIF) was found to be more inhibited (∼17%) in HepG2 cells in REC- 2006 + radiation-treated group. More inhibition (∼33%) of cytochrome c was observed in HepG2 cells upon REC-2006 treatment 2 h prior irradiation. Similarly, significantly more (P .05) inhibition of Apaf-1, caspase-9 and caspase-3 was observed in REC- 2006 + radition-treated group in HepG2 cells. REC-2006 treatment restored the expression of ICAD in HepG2 cells; however, no restoration was observed in Hep3B cells. Lower nuclear to cytoplasmic CAD ratio was observed in HepG2 cells (∼0.6) as compared