role of apoptosis in rabies viral encephalitis a comparative study in mice, canine, and human brain with a review of literature细胞凋亡在狂犬病病毒性脑炎的比较研究老鼠,狗和人类大脑的文学.pdfVIP

SAGE-Hindawi Access to Research Pathology Research International Volume 2011, Article ID 374286, 13 pages doi:10.4061/2011/374286 Research Article Role of Apoptosis in Rabies Viral Encephalitis: A Comparative Study in Mice, Canine, and Human Brain with a Review of Literature M. S. Suja,1 Anita Mahadevan,1 S. N. Madhusudana,2 and S. K. Shankar1 1 Department of Neuropathology, National Institute of Mental Health and Neurosciences (NIMHANS), Bangalore 560 029, India 2 Department Neurovirology, National Institute of Mental Health and Neurosciences (NIMHANS), Bangalore 560 029, India Correspondence should be addressed to Anita Mahadevan, anita mahadevan@ Received 16 October 2010; Accepted 9 June 2011 Academic Editor: C. Sundaram Copyright © 2011 M. S. Suja et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. To evaluate the role of apoptosis in rabies encephalitis in humans and canines infected with wild-type street virus, in comparison with rodent model infected with street and laboratory passaged CVS strain, we studied postmortem brain tissue from nine humans, six canines infected with street rabies virus, and Swiss albino mice inoculated intramuscularly (IM) and intracerebrally (IC) with street and CVS strains. Encephalitis and high rabies antigen load were prominent in canine and human brains compared to rodents inoculated with street virus. Neuronal apoptosis was detectable only in sucking mice inoculated with CVS strain and minimal in street virus inoculated mice. In a time point study in suckling mice, DNA laddering was noted only terminally (7 days p.i.) following IC inoculation with CVS strain but not with stre