schwann cell metabolic activity in various short-term holding conditions implications for improved nerve graft viability许旺细胞代谢活动在各种短期持有对改善神经移植物生存条件的影响.pdfVIP

Hindawi Publishing Corporation International Journal of Otolaryngology Volume 2012, Article ID 742183, 8 pages doi:10.1155/2012/742183 Research Article Schwann Cell Metabolic Activity in Various Short-Term Holding Conditions: Implications for Improved Nerve Graft Viability Insa Janssen, Kerstin Reimers, Christina Allmeling, Stella Matthes, Peter M. Vogt, and Christine Radtke Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, 30625 Hannover, Germany Correspondence should be addressed to Christine Radtke, radtke.christine@mh-hannover.de Received 17 July 2011; Revised 26 September 2011; Accepted 30 September 2011 Academic Editor: Peter S. Roland Copyright © 2012 Insa Janssen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Strategies for improvement of nerve regeneration and optimal conditions to prevent Schwann cell (SC) loss within a nerve transplant procedure are critical. The purpose of this study was to examine SC viability, which plays an important role in peripheral nerve regeneration, under various incubation conditions up to three hours. To address this issue, Schwann cell metabolic activity was determined using different independent test methods. The following experimental conditions were compared: SCs prepared from nerves were incubated in (1) isotonic saline solution (2) Dulbecco’s modified Eagles medium as used for cell culturing, (3) Hannover bioreactor medium, and (4) Leibovitz’s medium. SC metabolic activity of excised rat sciatic nerve was determined ◦ ◦ ◦ at 4 C, 18 C, and 37 C over 3 hrs. The results indicate that SC ac