olig2-induced neural stem cell differentiation involves downregulation of wnt signaling and induction of dickkopf-1 expressionolig2-induced神经干细胞分化dickkopf-1 wnt信号和归纳的差别是对这些基因的表达.pdfVIP

Olig2-Induced Neural Stem Cell Differentiation Involves Downregulation of Wnt Signaling and Induction of Dickkopf-1 Expression 1. 1. 1 1 2 3,4 Sung-Min Ahn , Kyunghee Byun , Deokhoon Kim , Kiyoung Lee , Jong Shin Yoo , Seung U. Kim *, 5 6 1 Eek-hoon Jho , Richard J. Simpson *, Bonghee Lee * 1 Center for Genomics and Proteomics, Lee Gil Ya Cancer and Diabetes Institute, Gachon University of Medicine and Science, Incheon, Korea, 2 Mass Spectrometry Analysis Group, Korea Basic Science Institute, Daejeon, Korea, 3 Gachon Institute for Regenerative Medicine, Gachon University of Medicine and Science, Incheon, Korea, 4 Department of Medicine, University of British Columbia, Vancouver, Canada, 5 Department of Life Science, The University of Seoul, Dongdaemun-gu, Seoul, Korea, 6 Joint Proteomics Laboratory, Ludwig Institute for Cancer Research the Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia Abstract Understanding stem cell-differentiation at the molecular level is important for clinical applications of stem cells and for finding new therapeutic approaches in the context of cancer stem cells. To investigate genome-wide changes involved in differentiation, we have used immortalized neural stem cell (NSC) line (HB1.F3) and Olig2-induced NSC differentiation model (F3.Olig2). Using microarray analysis, we revealed that Olig2-induced NSC differentiation involves downregulation of Wnt pathway, which was further confirmed by TOPflash/FOPflash reporter assay, RT-PCR analysis, immunoblots, and immunocytochemistry