olfactory proteins mediating chemical communication in the navel orangeworm moth, amyelois transitella嗅觉蛋白质调节化学通讯肚脐orangeworm蛾,amyelois transitella.pdfVIP

Olfactory Proteins Mediating Chemical Communication in the Navel Orangeworm Moth, Amyelois transitella 1 1 1 1 1 2 2 Walter S. Leal *, Yuko Ishida , Julien Pelletier , Wei Xu , Josep Rayo , Xianzhong Xu , James B. Ames 1 Department of Entomology, University of California Davis, Davis, California, United States of America, 2 Department of Chemistry, University of California Davis, Davis, California, United States of America Abstract Background: The navel orangeworm, Amyelois transitella Walker (Lepidoptera: Pyralidae), is the most serious insect pest of almonds and pistachios in California for which environmentally friendly alternative methods of control — like pheromone- based approaches — are highly desirable. Some constituents of the sex pheromone are unstable and could be replaced with parapheromones, which may be designed on the basis of molecular interaction of pheromones and pheromone- detecting olfactory proteins. Methodology: By analyzing extracts from olfactory and non-olfactory tissues, we identified putative olfactory proteins, obtained their N-terminal amino acid sequences by Edman degradation, and used degenerate primers to clone the corresponding cDNAs by SMART RACE. Additionally, we used degenerate primers based on conserved sequences of known proteins to fish out other candidate olfactory genes. We expressed the gene encoding a newly identified pheromone- binding protein, which was analyzed by circular dichroism, fluorescence, and nuclear magnetic resonance, and used in a binding assay to assess affinity to pheromone components. Conclusion: We have cloned nine cDNAs encoding olfactory proteins fro