sers microspectroscopy of biomolecules on dried ag colloidal dropsser显微镜生物分子ag)胶体滴干.pdfVIP

Hindawi Publishing Corporation Spectroscopy: An International Journal Volume 27 (2012), Issue 5-6, Pages 449–453 doi:10.1155/2012/393847 SERS Microspectroscopy of Biomolecules on Dried Ag Colloidal Drops ˇ ´ ´ ´ ˇ ˇ ´ P. Simakova, M. Prochazka, and E. Kocisova Institute of Physics, Faculty of Mathematics and Physics, Charles University, Ke Karlovu 5, 121 16 Prague 2, Czech Republic ˇ ´ ´ Correspondence should be addressed to P. Simakova, simakova@karlov.mff.cuni.cz ˇ ´ ´ Copyright © 2012 P. Simakova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract. We report the application of dried Ag hydroxylamine-reduced colloidal drops to surface-enhanced (resonance) Raman scattering (SE(R)RS) study of biomolecules using Raman microspectroscopy. 5,10,15,20-tetrakis(1-methyl-4- pyridyl)porphyrin (TMPyP), amino acid tryptophan, and phospholipid 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) served as testing biomolecules. Ag colloid/biomolecule drop dried on glass support forms a ring in the edge part of the drop in which almost all nanoparticles are clustered. This specific drying process promotes adsorption of the studied biomolecules in highly enhancing sites (“hot spots”) as well as concentrates them in the ring. We were able to obtain SE(R)RS spectra from the ring that cannot be acquired directly from Ag colloidal solutions (SERRS spectrum of 1 × 10−10 M TMPyP by 1 s accumulation time, SERS spectrum of 2 × 10−7 M DSPC). Despite the spectral irreproducibility and problems with spurious bands in some cases, SERS microspec