suppressive effect of juzentaihoto on vascularization induced by b16 melanoma cells in vitro and in vivo抑制juzentaihoto对黑色素瘤b16转椅引起的血管化的影响细胞在体外和体内.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2012, Article ID 945714, 9 pages doi:10.1155/2012/945714 Research Article Suppressive Effect of Juzentaihoto on Vascularization Induced by B16 Melanoma Cells In Vitro and In Vivo Shintaro Ishikawa, Takako Ishikawa, Kazuhito Asano, Hiroshi Fujiwara, Mayumi Okada, Masataka Sunagawa, and Tadashi Hisamitsu Department of Physiology, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan Correspondence should be addressed to Shintaro Ishikawa, s-ishikawa@med.showa-u.ac.jp Received 20 June 2011; Accepted 23 August 2011 Academic Editor: Yoshiyuki Kimura Copyright © 2012 Shintaro Ishikawa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Juzentaihoto (JTT) is well known to be one of Japanese herbal medicines, and used for the supplemental therapy of cancer patients with remarkable success. The present study, therefore, was undertaken to examine the possible therapeutic mechanisms of JTT on cancer using B16 melanoma cell (B16 cell)/experimental mouse system. JTT was well mixed with rodent chow at 3.0% concentrations, and was administered orally ad libitum. Administration of JTT was started one week before tumor cell injection and continued throughout the experiment. Administration of JTT into mice significantly inhibited tumor metastasis in lungs after intravenous injection of 2 × 105 B16 cells in a volume of 50 μL. JTT also significantly suppressed enlargement of tumor size in hind footpad after the subcutaneous injection of 2 × 105 (50μL) B16 cells. In the