the cytotoxicity mechanism of 6-shogaol-treated hela human cervical cancer cells revealed by label-free shotgun proteomics and bioinformatics analysis6-shogaol-treated海拉人宫颈癌细胞的细胞毒性机制揭示了label-free猎枪蛋白质组学和生物信息学分析.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2012, Article ID 278652, 12 pages doi:10.1155/2012/278652 Research Article The Cytotoxicity Mechanism of 6-Shogaol-Treated HeLa Human Cervical Cancer Cells Revealed by Label-Free Shotgun Proteomics and Bioinformatics Analysis Qun Liu, Yong-Bo Peng, Lian-Wen Qi, Xiao-Lan Cheng, Xiao-Jun Xu, Le-Le Liu, E-Hu Liu, and Ping Li State Key Laboratory of Natural Medicines, Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210009, China Correspondence should be addressed to E-Hu Liu, liuehu2011@163.com and Ping Li, liping2004@126.com Received 26 July 2012; Revised 24 September 2012; Accepted 1 October 2012 Academic Editor: Shrikant Anant Copyright © 2012 Qun Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Cervical cancer is one of the most common cancers among women in the world. 6-Shogaol is a natural compound isolated from the rhizome of ginger (Zingiber offi cinale). In this paper, we demonstrated that 6-shogaol induced apoptosis and G2/M phase arrest in human cervical cancer HeLa cells. Endoplasmic reticulum stress and mitochondrial pathway were involved in 6-shogaol- mediated apoptosis. Proteomic analysis based on label-free strategy by liquid chromatography chip quadrupole time-of-flight mass spectrometry was subsequently proposed to identify, in a non-target-biased manner, the molecular changes in cellular proteins in response to 6-shogaol treatment. A total of 287 proteins were differentially expressed in response to 24 h treatment with 15 μM 6- shogaol in HeLa cells. Significantly changed proteins were