the pol3-t hyperrecombination phenotype and dna damage-induced recombination in saccharomyces cerevisiae is rad50 dependent的pol3-t hyperrecombination表型和dna损害在酿酒酵母重组rad50依赖.pdfVIP

Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Volume 2009, Article ID 312710, 9 pages doi:10.1155/2009/312710 Research Article The pol3-t Hyperrecombination Phenotype and DNA Damage-Induced Recombination in Saccharomyces cerevisiae Is RAD50 Dependent Alvaro Galli,1 Kurt Hafer,2 Tiziana Cervelli,1 and Robert H. Schiestl2 1 Laboratory of Gene and Molecular Therapy, Institute of Clinical Physiology, CNR, 56124 Pisa, Italy 2 Departments of Pathology, Radiation Oncology, and Environmental Health, UCLA School of Medicine, Los Angeles, CA 90095, USA Correspondence should be addressed to Alvaro Galli, alvaro.galli@ifc.cnr.it Received 25 May 2009; Accepted 21 July 2009 Recommended by Celina Janion The DNA polymerase δ (POL3/CDC2) allele pol3-t of Saccharomyces cerevisiae has previously been shown to be sensitive to methylmethanesulfonate (MMS) and has been proposed to be involved in base excision repair. Our results, however, show that the pol3-t mutation is synergistic for MMS sensitivity with MAG1, a known base excision repair gene, but it is epistatic with rad50Δ, suggesting that POL3 may be involved not only in base excision repair but also in a RAD50 dependent function. We further studied the interaction of pol3-t with rad50Δ by examining their effect on spontaneous, MMS-, UV-, and ionizing radiation- induced intrachromosomal recombination. We found that rad50Δ completely abolishes the elevated spontaneous frequency of intrachromosomal recombination in the pol3-t mutant and significantly decreases UV- and MMS-induced recombination in both POL3 and pol3-t strains. Interestingly, rad50Δ had no effect on γ-ray-induced recombination in both backgrounds between 0 and 50 Gy. Finally, the deletion of RAD50 had no effect on the elevate